Journal: International Journal of Health Sciences
Article Title: Novel predication of protein biomarkers in interferon-gamma-stimulated breast cancer cells
doi:
Figure Lengend Snippet: Representative two-dimensional gel images of 3,4-methylenedioxyamphetamine-MB-231 total cell proteins from cells untreated and treated with interferon-gamma (IFN-γ) (a) Control (untreated) cells only in 10% FCS medium. (b) Cells treated with 100 IU/ml IFN-γ. (c) Cells treated with 500 IU/ml IFN-γ. (d) Cells treated with 1000 IU/ml IFN-γ. Incubations were for 72 h. In the first dimension, 80 μg total soluble protein was separated on Immobiline IPG strips (18 cm, pH 3–10 NL). Isoelectric focusing was performed on an IPGPhor unit. The second dimension was performed on 12% SDS-PAGE gels, with image analysis carried out with Progenesis SameSpot software. M - Molecular mass standards are shown on the left side (kDa - kiloDaltons). Differentially expressed, user selected protein spots in four gels are marked by red arrows
Article Snippet: Scanned gel images were characterized with Progenesis SameSpot software package (Nonlinear Dynamics Limited, UK).
Techniques: Two-Dimensional Gel Electrophoresis, Control, SDS Page, Software